Composite

Part:BBa_K3329063

Designed by: Wenfei Xiao   Group: iGEM20_BNDS_China   (2020-10-20)


SpyTag003*3-spider fibroin*2-sfGFP for P. Pastoris

SpyTag003 is a sub part in the Spytag-catcher003 system. It should be used with Spycatcher003. The super-folder GFP protein is connected with spider fibroin. The green color indicates whether spider fibroin is bound to cellulose by seeing whether that filter paper turned green.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 586
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1270


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Categories
Parameters
None