Coding

Part:BBa_K3329050

Designed by: Wenfei Xiao   Group: iGEM20_BNDS_China   (2020-10-13)


mRFP optimized V1

The monomeric red fluorescent protein is connected with CBM. The red color indicates whether the CBM is bound to cellulose by seeing whether that filter paper turned red.

This part is codon optimized for the expression in P. Pastoris.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 430
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None