Part:BBa_K3329023
CBM optimized V2
The cellulose binding module (CBM) help connect cellulose and Spycatcher003.
It's noteworthy that, in our Protein Crosslinking design, we intended to use double CBM in order to anchor the CBM and SpyCatcher003 firmly. Moreover, CBM would be expressed in both P. Pastoris and E. coli, respectively. Therefore, codon optimization must be applied in order to avoid homologous recombination and fit the chassis organism. As a result, we synthesized 4 versions of genes coding the same CBM sequence based on the 2 versions of codon optimization for P. Pastoris and 2 versions for the codon optimization of E. coli. Names for those 4 versions of CDS are documented from CBM optimized V1 to CBM optimized V4, in which the CBM optimized V1 and V2 are for P. Pastoris, and CBM optimized V3-4 are for E. coli.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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