Composite

Part:BBa_K332021

Designed by: Chen-Hsi Tang   Group: iGEM10_NCTU_Formosa   (2010-10-24)

Ptet + RBS + LuxR

Ptet is a promoter which is negative-regulated by tetR. Without tetR, Ptet begins to work and the following proteins are produced. Add other useful parts and teminator behind K332022, it can become a intact functional unit. LuxI can convert SAM, a metabolite produced by E.coli, to AHL. With luxR protein, AHL and luxR form a complex to activate Plux. Plux detect the concentration of AHL-LuxR complex and initiate the transcription of following gene. To know more about how we have used this part, click the part design to get more information and also search the part K332022.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/transcriptionalregulator/activator
//chassis/prokaryote/ecoli
//function/regulation/transcriptional
//regulation/positive
Parameters
control
positive_regulators
proteins