Composite
Part:BBa_K3320019
Designed by: Joseana Mendes Comodaro Group: iGEM19_Amazonas-Brazil (2019-10-20)
Lactate Repressor Unit
This is a part from our AND Gate, called lactate repressor unit.
Usage and Biology
The lactate repressor unit is composed of the lactate promoter, inducible in the presence of lactate, producing the PhlF repressor protein. This protein represses the PhlF promoter, which is responsible for producing the Tet repressor protein, that regulates the expression of BBa_K3320013 by Tet promoter.
Figure 1. BBa_K3320019 as insert.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 965
Illegal NheI site found at 988 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 614
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3284
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Categories
Parameters
None |