Composite

Part:BBa_K3320019

Designed by: Joseana Mendes Comodaro   Group: iGEM19_Amazonas-Brazil   (2019-10-20)


Lactate Repressor Unit

This is a part from our AND Gate, called lactate repressor unit.

Usage and Biology

The lactate repressor unit is composed of the lactate promoter, inducible in the presence of lactate, producing the PhlF repressor protein. This protein represses the PhlF promoter, which is responsible for producing the Tet repressor protein, that regulates the expression of BBa_K3320013 by Tet promoter.

BBa K3320019 Map.png

Figure 1. BBa_K3320019 as insert.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 965
    Illegal NheI site found at 988
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 614
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3284


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Categories
Parameters
None