Composite

Part:BBa_K3320018

Designed by: Joseana Mendes Comodaro   Group: iGEM19_Amazonas-Brazil   (2019-10-20)


pSrp + TetR

Second part of the hypoxia repressor unit from our AND Gate.

Usage and Biology

It’s an orthogonal repressed promoter by the SrpR protein. In our circuit, this part constitutively produces TetR, which, in our circuit, represses Tet promoter in the absence of hypoxia.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 20
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None