Composite
Part:BBa_K3320018
Designed by: Joseana Mendes Comodaro Group: iGEM19_Amazonas-Brazil (2019-10-20)
pSrp + TetR
Second part of the hypoxia repressor unit from our AND Gate.
Usage and Biology
It’s an orthogonal repressed promoter by the SrpR protein. In our circuit, this part constitutively produces TetR, which, in our circuit, represses Tet promoter in the absence of hypoxia.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 20
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |