Composite

Part:BBa_K3320007

Designed by: Joseana Mendes Comodaro   Group: iGEM19_Amazonas-Brazil   (2019-10-19)


Alpha-hemolysin secretion system

This is a type I secretion system, used for protein translocation.


Usage and Biology

The promoter J23111 constitutively produces the alpha-hemolysin secretion system from Escherichia coli. The system is composed of HlyB, an internal membrane protein, HlyD, a membrane fusion protein, and TolC, an outer membrane protein. To use this secretion system, you should remind to add HlyA C-terminal sequence to the molecule you wish to secret. HlyA connects to the complex HlyB-HlyD, which induces contact with TolC, creating a periplasmic export channel.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1344
    Illegal BglII site found at 2007
    Illegal BamHI site found at 4051
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 274
    Illegal AgeI site found at 3511
    Illegal AgeI site found at 3568
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3903


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Categories
Parameters
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