Composite

Part:BBa_K3320003

Designed by: Isaac de Paula Guerreiro   Group: iGEM19_Amazonas-Brazil   (2019-10-16)


pTet + Ellowitz RBS + GFPMut3 + L3S2P22

This composite part produce GFPMut3 induced by pTet promoter.

Usage and Biology

This part is repressed by BBa_K3320019 and K3320016 in absence of Lactate and Hypoxia, physiological characteristics from the tumor microenvironment. When induced, it expresses the BBa_K3320013 to be secreted directly from the cytosol into the extracellular environment by the alpha-hemolysin secretion system.

Assembly

This part was cloned on the pUC19 vector, totaling 3752 bp.

BBa K3320003 Map.png

Figure 1. BBa_K3320003 plasmidial map

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None