Composite
Part:BBa_K3320003
Designed by: Isaac de Paula Guerreiro Group: iGEM19_Amazonas-Brazil (2019-10-16)
pTet + Ellowitz RBS + GFPMut3 + L3S2P22
This composite part produce GFPMut3 induced by pTet promoter.
Usage and Biology
This part is repressed by BBa_K3320019 and K3320016 in absence of Lactate and Hypoxia, physiological characteristics from the tumor microenvironment. When induced, it expresses the BBa_K3320013 to be secreted directly from the cytosol into the extracellular environment by the alpha-hemolysin secretion system.
Assembly
This part was cloned on the pUC19 vector, totaling 3752 bp.
Figure 1. BBa_K3320003 plasmidial map
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |