Composite

Part:BBa_K3320002

Designed by: Isaac de Paula Guerreiro   Group: iGEM19_Amazonas-Brazil   (2019-10-16)


phiF + RBS + tetR + L3S32P21

This composite part produces TetR protein induced by the phlF promoter.

Usage and Biology

This part expresses the tetR protein, which regulates the expression of BBa_K3320013 by Tet promoter. The phlF promoter is repressed by PhlF repressor from the BBa_K3320014 in absence of lactate.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None