Composite

Part:BBa_K3316009

Designed by: Luana Langlois   Group: iGEM19_Western_Canada   (2019-10-12)


Laccase-SpyTag

Fusion protein product containing E. coli K12 Lacccase (CueO), a copper-centric enzyme that catalyzes the oxidation of polyphenolic compounds, linked by a 12 amino acid GS linker to the SpyTag moiety. The coding sequence is preceded by the 5' UTR and first 47 amino acids of the E. coli type III secretion system protein flagellin, FliC47 to promote extracellular transport of the protein product. A 6-His tag was added at the C-terminus of Cutinase to facilitate protein isolation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 442
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None