Composite

Part:BBa_K3316008

Designed by: Luana Langlois   Group: iGEM19_Western_Canada   (2019-10-12)


SpyTag-Laccase

Fusion protein product containg the SpyTag moiety linked by a 12 amino acid GS linker to E. coli K12 Lacccase (CueO), a copper-centric enzyme that catalyzes the oxidation of polyphenolic compounds. The coding sequence is preceded by the 5' UTR and first 47 amino acids of the E. coli type III secretion system protein flagellin, FliC47 to promote extracellular transport of the protein product. A 6-His tag was added at the C-terminus of Cutinase to facilitate protein isolation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 533
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None