Composite

Part:BBa_K3316006

Designed by: Luana Langlois   Group: iGEM19_Western_Canada   (2019-10-12)


SpyTag-Cutinase

Fusion protein product containg the SpyTag moiety linked by a 12 amino acid GS linker to Cutinase (CueO), a cuticula degrading hydrolase from the fungus Fusarium solani pisi. The coding sequence is preceded by the 5' UTR and first 47 amino acids of the E. coli type III secretion system protein flagellin, FliC47 to promote extracellular transport of the protein product. A 6-His tag was added at the C-terminus of Cutinase to facilitate protein isolation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 687
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 833
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 609


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Categories
Parameters
None