Composite

Part:BBa_K3316005

Designed by: Luana Langlois   Group: iGEM19_Western_Canada   (2019-10-12)


CsgA-SpyCatcher

Coding sequence of fusion protein product containg the SpyCatcher moiety linked by a 13 amino acid GS linker to the major curlin fibre precursor subunit, CsgA. Sequence is preceded by a 6-His tag.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 371
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 371
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 371
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 371
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None