Deleted
Experience: None
Not Used
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Composite

Part:BBa_K331015

Designed by: Lisza Bruder   Group: iGEM10_Lethbridge   (2010-07-01)


IPTG Inducible Catechol 1,2-dioxygenase with C-terminal Arginine Tag

This part is IPTG inducible to produce catechol 1,2-dioxygenase with an C-terminal arginine tag. The arginine sequence allows for the targeting of ECFP into the Lumazine Synthase microcompatment (Bba_K249003).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 364
    Illegal BamHI site found at 652
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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