Intermediate

Part:BBa_K3309002

Designed by: Thibault Fillion   Group: iGEM19_Orleans   (2019-10-15)


Cupriavidus metallidurans CH34 PbrR 300 nucleotides downstream region

this part is the 304 bp downstream region of pbrR gene from Cupriavidus metallidurans CH34 and is intended to be used, along with BBa_K3309003 in homologous recombination on pbrR. It include the last 17 nucleotides from pbrR ORF, consequently, recombination souhld append inside of the ORF. A way to use BBa_K3309002 and BBa_K3309003 for homogous recombination in Cupriavidus metallidurans CH34 is to insert them inside of pCM184 vector (source). The resulting vector can be used to replace the inside of pbrR ORF with a kanamycin resistance gene ORF surrounded with loxP sites.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 71
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 189


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Categories
Parameters
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