Regulatory

Part:BBa_K3309000

Designed by: Thibault Fillion   Group: iGEM19_Orleans   (2019-10-15)


pan promoter

pan is a strong and heavy metal repressed promoter from Bacillus subtilis. Used in cupriavidus metallidurans CH32, it becomes heavy metal inducible, with a strong basal expression. It is induced by cadmium and lead.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 429
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 429
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 429
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 429
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 429
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None