Part:BBa_K3301013
Codon optimized B. theta gene BT2269 with 6xHis tag and T7 promoter.
This construct contains the BT2269 gene that has been codon optimized for E. coli. The gene, originating from the symbiotic gut bacteria Bacteroides thetaiotaomicron, produces a protein of the Mucus-Associated Functional Factor (MAFF) molecular family. In the microbiome, this protein is only expressed when B. theta is embedded in the mucosal lining and coated with IgA. Our project sought to remove the gene from this restricted environment and enable its production in oxygen-tolerant E. coli, regulated by an inducible T7 promoter. This would enable production of the protein on an as-needed basis, to serve as a supplement to the microbiome after it is depleted by illness or antibiotics. A 6xHis tag was added for protein isolation and detection.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1050
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |