Part:BBa_K3301002
Codon optimized B. theta gene BT2269
This construct contains the Bacteroides thetaiotaomicron BT2269 gene that has been codon optimized for E. coli. It's suggested to secrete an outer membrane protein that aids in starch digestion, which also promotes diversity when present in the human microbiome. The protein is known to be a SusD homolog that serves to break long starches into smaller chains that can pass through the nutrient channel formed by BT2268 (BBa_K3301001), a SusC homolog. When removed from B. theta, the cell’s growth and proliferation slow dramatically (Nakajima et al., 2019). In the intestinal mucosal lining, its production is primarily regulated by IgA secretion. This is the gene directly from B. theta, codon optimized for E. coli, with some restriction sites edited and the signal sequence removed.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 998
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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