Part:BBa_K3301001

Codon optimized B. theta gene BT2268

This construct contains the Bacteroides thetaiotaomicron BT2268 gene that has been codon optimized for E. coli. It's suggested to secrete a membrane protein that aids in starch digestion, which also promotes diversity when present in the human microbiome. The protein is known to be a SusC homolog that serves as a channel to admit food into the cell. It pairs with BT2269 (BBa_K3301002), a SusD homolog, to increase the cell’s nutrient absorption. When removed from B. theta, the cell’s growth and proliferation slow dramatically (Nakajima et al., 2019). In the intestinal mucosal lining, its production is primarily regulated by IgA secretion. This is the gene directly from B. theta, codon optimized for E. coli, with some restriction sites edited and the signal sequence removed.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1735
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1178
  Illegal BsaI.rc site found at 1634
  Illegal SapI site found at 1553