Device

Part:BBa_K329009

Designed by: Raphael Pantier   Group: iGEM10_Paris_Liliane_Bettencourt   (2010-10-12)


attC - double terminator (B0015) - attC - mRFP

This part is composed of a double terminator (BBa_B0015) flanked by attC sites and followed by mRFP (with an RBS). When the IntI integrase is expressed (and if there is a promoter upstream of this part), it can specifically excise the terminator and triggers the expression of mRFP.

Important note : RBS-mRFP is situated between the SpeI and PstI restriction sites, the excision of this gene permit to restore a normal biobrick suffix.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 277
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 277
    Illegal NotI site found at 1153
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 277
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 277
    Illegal AgeI site found at 856
    Illegal AgeI site found at 968
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None