DNA

Part:BBa_K3289013:Design

Designed by: Tatiana Houhou   Group: iGEM19_NYU_Abu_Dhabi   (2019-10-21)


IS481 for the Detection of Whooping Cough


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 94
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When we ordered the gblock from IDT, we requested that it is 5' end phosphorylated to facilitate its blunt end ligation into pJET backbone vector which is then transformed into E coli. for DNA cloning.


Source

Bordetella Pertussis

References

(1) Finger, Horst. “Bordetella.” Medical Microbiology. 4th Edition., U.S. National Library of Medicine, 1 Jan. 1996, www.ncbi.nlm.nih.gov/books/NBK7813/.

(2) NHS Choices, NHS, www.nhs.uk/conditions/whooping-cough/.

(3) Brown, Jennifer J. “10 Essential Facts About Whooping Cough.” EverydayHealth.com, Everyday Health, 30 June 2015, www.everydayhealth.com/news/10-essential-facts-about-whooping-cough/.

(4) GBD 2015 Mortality and Causes of Death Collaborators. “Global, Regional, and National Life Expectancy, All-Cause Mortality, and Cause-Specific Mortality for 249 Causes of Death, 1980-2015: a Systematic Analysis for the Global Burden of Disease Study 2015.” Lancet (London, England), Elsevier, 8 Oct. 2016, www.ncbi.nlm.nih.gov/pmc/articles/PMC5388903/.

(5) “Pertussis.” World Health Organization, World Health Organization, 13 Aug. 2013, www.who.int/ith/diseases/pertussis/en/.

(6) “Pertussis | Whooping Cough | Clinical | Disease Specifics | CDC.” Centers for Disease Control and Prevention, Centers for Disease Control and Prevention, www.cdc.gov/pertussis/clinical/disease-specifics.html.

(7) “Whooping Cough - Symptoms, Treatment, Vaccination.” Whooping Cough - Symptoms, Treatment, Vaccination - Southern Cross NZ, www.southerncross.co.nz/group/medical-library/whooping-cough-symptoms-treatment-vaccination.

(8) van der Zee, Anneke, et al. “Laboratory Diagnosis of Pertussis.” Clinical Microbiology Reviews, American Society for Microbiology, Oct. 2015, www.ncbi.nlm.nih.gov/pmc/articles/PMC4575397/#B131.

(9) McLafferty, M A, et al. “Nucleotide Sequence and Characterization of a Repetitive DNA Element from the Genome of Bordetella Pertussis with Characteristics of an Insertion Sequence.” Journal of General Microbiology, U.S. National Library of Medicine, Aug. 1988, www.ncbi.nlm.nih.gov/pubmed/2908119.

https://www.ncbi.nlm.nih.gov/pubmed/2908119