DNA

Part:BBa_K3289011:Design

Designed by: Tatiana Houhou   Group: iGEM19_NYU_Abu_Dhabi   (2019-10-21)


pcAa for the Detection of Tuberculosis


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 25
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 25
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 25
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 25
    Illegal NgoMIV site found at 366
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When we ordered the gblock from IDT, we requested that is 5'end phosphorylated to facilitate the blunt end ligation into pJET backbone vector which was transformed into E coli. for DNA cloning.


Source

Mycobacterium Tubercolosi

References

Glaziou, Philippe et al. “Global epidemiology of tuberculosis.” Cold Spring Harbor perspectives in medicine vol. 5,2 a017798. doi:10.1101/cshperspect.a017798

https://www.tbfacts.org/tb-statistics/

Rao, Vivek et al. “Mycobacterium tuberculosis controls host innate immune activation through cyclopropane modification of a glycolipid effector molecule.” The Journal of experimental medicine vol. 201,4 (2005): 535-43. doi:10.1084/jem.20041668

https://www.ncbi.nlm.nih.gov/gene?term=(pcaa[gene])%20AND%20(Mycobacterium%20tuberculosis%20H37Rv[orgn])%20AND%20alive[prop]%20NOT%20newentry[gene]&sort=weight