Coding
Part:BBa_K3286283
Designed by: Santiago Castanedo Fontanillas Group: iGEM19_Wageningen_UR (2019-10-21)
Lambda gpD - ribosomal frameshifting sequence - Chitinase A1
This part can be used for the constitutive expression of a fusion gene that cosists of : - 6X-His tag at the N-terminus of the protein, used to quantify the amount of expressed fusion protein via Western blot. - gpd gene (encodes the lambda decorator protein) from the lambda phage. - A ribosomal frameshifting sequence that links the two proteins together. - Chitinase A gene so that eventually, if engineered with this fusion protein, phages can display the fusion protein on the capsid and therefore, phages can bind to chitin. The promoter is very strong (J23100), as well as the ribosome binding site (RBS).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |