Coding

Part:BBa_K3286282

Designed by: Santiago Castanedo Fontanillas   Group: iGEM19_Wageningen_UR   (2019-10-21)


Lambda gpD - linker - Chitinase A1

This part can be used for the constitutive expression of a fusion gene that cosists of : - 6X-His tag at the N-terminus of the protein, used to quantify the amount of expressed fusion protein via Western blot. - gpd gene (encodes the lambda decorator protein) from the lambda phage. - A small linker C(GS)3G that fuses the two proteins. - Chitinase A gene so that eventually, if engineered with this fusion protein, phages can display the fusion protein on the capsid and therefore, phages can bind to chitin.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None