DNA

Part:BBa_K3286278

Designed by: Marijn Ceelen   Group: iGEM19_Wageningen_UR   (2019-10-21)


Lambda gpD - GFP recombination module

This part can be used in combination with the lambda red system to insert a fusion of capsid decorator protein D and GFP into the phage lambda genome. The function of this is to create a bacteriophage that is fluorescent. The part should be used in combination with a plasmid carrying the lambda red system, which increases recombination. Cells carrying this combination should be infected with bacteriophage lambda. A fraction of the resulting bacteriophages will carry the fusion protein gene. To select for the recombinant phages a CRISPR-Cas counterselection system can be used, either in the first step or in a separate counterselection step.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1210


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