Coding

Part:BBa_K3286273

Designed by: Marijn Ceelen   Group: iGEM19_Wageningen_UR   (2019-10-21)


Phage T7 P10 - Chitinase A fusion

This part allows you to create a fusion protein that combines capsid protein P10 from phage T7 with the chitin binding domain from Chitinase A. This can theoretically be used to create phages that bind to chitin, a common insect protein. The fusion protein is under the control of the T7 promoter, in exactly the same way as the original T7 capsid protein. This should lead to a mix of wild type T7 capsid protein and fusion protein. This choice was made to avoid overexpression of the capsid fusion, which might make the phage unviable.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 84
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 372
    Illegal BsaI.rc site found at 20
    Illegal BsaI.rc site found at 576


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Parameters
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