Composite

Part:BBa_K3286272

Designed by: Marijn Ceelen   Group: iGEM19_Wageningen_UR   (2019-10-21)


Lambda gpD - PD1764 fusion

This part allows you to create a fusion protein that combines the capsid decorator protein from phage lambda with the chitin binding protein PD1764. This can theoretically be used to create phages that bind to chitin, a common insect protein. The fusion protein is under the control of Anderson promoter J23116. This is a relatively weak Anderson promoter at 0.16 strength. This choice was made to avoid overexpression of the capsid fusion, which might make the phage unviable. It also contains a linker consisting of glycines and serines. This should help folding of the protein and increase phage capsid stability

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1251
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 758
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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