Coding

Part:BBa_K3286269

Designed by: Marijn Ceelen   Group: iGEM19_Wageningen_UR   (2019-10-21)


Lambda gpD - Chitinase A Fusion

This part allows you to create a fusion protein that combines the capsid decorator protein from phage lambda to the chitin binding domain from Chitinase A. This can theoretically be used to create phages that bind to chitin, a common insect protein. The fusion protein is under the control of Anderson promoter J23116. This is a relatively weak Anderson promoter at 0.16 strength. This choice was made to avoid overexpression of the caspsid fusion, which might make the phage unviable.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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