Coding
Part:BBa_K3286269
Designed by: Marijn Ceelen Group: iGEM19_Wageningen_UR (2019-10-21)
Lambda gpD - Chitinase A Fusion
This part allows you to create a fusion protein that combines the capsid decorator protein from phage lambda to the chitin binding domain from Chitinase A. This can theoretically be used to create phages that bind to chitin, a common insect protein. The fusion protein is under the control of Anderson promoter J23116. This is a relatively weak Anderson promoter at 0.16 strength. This choice was made to avoid overexpression of the caspsid fusion, which might make the phage unviable.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |