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Part:BBa_K3286267

Designed by: Marijn Ceelen   Group: iGEM19_Wageningen_UR   (2019-10-21)


phage Lambda gpD insertion homology region 2

This part can be used together with phage Lambda gpD insertion homology region 1 to insert regions in the phage Lambda genome. Phage Lambda is known to have high recombination rate [source]. To improve the recombination rate the Lambda red system can be used [source]. To select for recombinants a CRISPR-Cas counterselection system is recommended [source].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None