Coding

Part:BBa_K3286265

Designed by: Marijn Ceelen   Group: iGEM19_Wageningen_UR   (2019-10-21)


Phage T7 P10 without stop codon + translation initiation sequence + T7 promoter

Phage T7 capsid major capsid protein P10, including the T7 promoter and translation initiation sequence taken directly from the T7 genome. The stop codon has been removed to make the part applicable to protein fusions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 84
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 372
    Illegal BsaI.rc site found at 20
    Illegal BsaI.rc site found at 576


[edit]
Categories
Parameters
None