Composite

Part:BBa_K3286216

Designed by: Ben Kuipers   Group: iGEM19_Wageningen_UR   (2019-10-21)

rhamnose-inducible rhaBAD promoter + kaiABC Operon

The following composite parts contains the cyanobacterial circadian oscillator core genes, expressed in an operon driven by the rhamnose-inducible rhaBAD promoter (PrhaBAD) [1]. The core circadian clock of the cyanobacterium Synechococcus elongatus PCC 7942 is composed of three components, KaiA, KaiB, and KaiC, expressed as monocistronic kaiA and dicistronic kaiBC transcripts. The kaiBC operon is upregulated by the KaiA protein and downregulated by the KaiC protein to generate a circadian oscillator. KaiB can bind to KaiA, therefore opposing KaiA’s stimulatory activity by sequestering it, and to KaiC, driving the KaiC phosphorylation cycle in a clockwise direction.

  1. Chen, AH., Lubkowicz, D., Yeong, V., Chang, RL., and Silver, PA. (2015) Transplantability of a circadian clock to a noncircadian organism. Sci. Adv. 1, e1500358.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 559
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1307
    Illegal BamHI site found at 2220
    Illegal XhoI site found at 2287
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2943
    Illegal AgeI site found at 1039
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2350
[edit]
Categories
Parameters
None