Part:BBa_K3286044

Bacteriophage Lambda PL, PRM and PR promoters, operators and 5' UTR's

The native bacteriophage Lambda promoters (PL, PRM and PR), operator regions (OL1,2,3 and OR1,2,3) and 5' UTR (with RNase III sensitive hairpin-loop and NutL site). The PL and PR promoter can be regulated by the CI and the CRO protein, which is a native lambda transcribed protein.

Atsumi, S., & Little, J. W. (2004). Regulatory circuit design and evolution using phage λ. Genes and Development, 18(17), 2086–2094. https://doi.org/10.1101/gad.1226004

Court, D. L., Oppenheim, A. B., & Adhya, S. L. (2007). A new look at bacteriophage lambda genetic networks. Journal of Bacteriology, 189(2), 298–304.

Liu, X., Jiang, H., Gu, Z., & Roberts, J. W. (2013). High-resolution view of bacteriophage lambda gene expression by ribosome profiling. Proceedings of the National Academy of Sciences, 110(29), 11928–11933. https://doi.org/10.1073/pnas.1309739110

Ptashne, M. (2004). A genetic switch : phage lambda revisited. In Cold Spring Harbor Laboratory Press Cold Spring Harbor NY (Vol. 3).

Wilson, H. R., Kameyama, L., Zhou, J. G., Guarneros, G., & Court, D. L. (1997). Translational repression by a transcriptional elongation factor. Genes and Development, 11(17), 2204–2213. https://doi.org/10.1101/gad.11.17.2204

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]