Part:BBa_K3286042

Native bacteriophage Lambda PL promoter, OL operators and 5' UTR

The native bacteriophage Lambda PL promoter, OL operators (OL1, OL2 and OL3) and 5' UTR (with RNase III sensitive hairpin-loop and NutL site). The PL promoter can be regulated by the CI and the CRO protein originating from bacteriophage lambda.

References

Atsumi, S., & Little, J. W. (2004). Regulatory circuit design and evolution using phage λ. Genes and Development, 18(17), 2086–2094. https://doi.org/10.1101/gad.1226004

Court, D. L., Oppenheim, A. B., & Adhya, S. L. (2007). A new look at bacteriophage lambda genetic networks. Journal of Bacteriology, 189(2), 298–304.

Liu, X., Jiang, H., Gu, Z., & Roberts, J. W. (2013). High-resolution view of bacteriophage lambda gene expression by ribosome profiling. Proceedings of the National Academy of Sciences, 110(29), 11928–11933. https://doi.org/10.1073/pnas.1309739110

Ptashne, M. (2004). A genetic switch : phage lambda revisited. In Cold Spring Harbor Laboratory Press Cold Spring Harbor NY (Vol. 3).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]