Composite

Part:BBa_K3286003

Designed by: Paul Alexander Niederau   Group: iGEM19_Wageningen_UR   (2019-10-13)


sgRNA expression module

Expression module of the sgRNA scaffold for Cas9 of Streptococcus pyogenes with non-targeting (NT) spacer Part:BBa_K3286001 and rho-independent terminator Part:BBa_K3286002 under control of the strong constitutive Anderson promoter J23109 Part:BBa_J23119.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None