Coding

Part:BBa_K3285001

Designed by: Sayantan Datta   Group: iGEM19_IISER-Pune-India   (2019-10-15)


Temperature sensitive repressor of cI(lambda) promoter

The sequence codes for a protein, cIts2 which is a temperature sensitive mutant of cI. It belongs to a class of temperature sensitive repressor of the cI-lam promoter. The coding region is followed by a ssrA tag (LVA) which is a degradation tag. This mutant protein has a single amino acid change (224 K -> E) which is conjectured to cause problems in homodimerization of the protein affecting its overall activity. This is however still open to experimental verification. The protein has however been shown to have lower activity than the wild type cI protein at high temperatures mainly. (Ref:- https://academic.oup.com/peds/article/12/3/225/1563912).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None