Regulatory

Part:BBa_K3282006

Designed by: Manjyot Kaur, Mateusz Piotr Szewczyk   Group: iGEM19_Lund   (2019-10-11)


Tac Promoter

Tac is a hybrid promoter, which has the consensus -35 sequence from the trp promoter and the consensus -10 sequence from the lac UV5 promoter. It is reported to be 5-10 times stronger than the lac UV5 promoter and is induced using IPTG and other galactosides while not being subject to catabolite repression (1). The higher efficiency of this hybrid promoter with respect to either one of the parental promoters is attributed to the presence of consensus Pribnow box sequence in addition to consensus -35 sequence (2). This part was used in composite parts BBa_K3282004 and BBa_K3282007

References

1. Donovan, R.S., Robinson, C.W. & Glick, B.R. Journal of Industrial Microbiology (1996) 16: 145.

2. de Boer, H. A., Comstock, L. J., & Vasser, M. (1983). The tac promoter: a functional hybrid derived from the trp and lac promoters. Proceedings of the National Academy of Sciences of the United States of America, 80(1), 21–25.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None