Plasmid_Backbone

Part:BBa_K3265035

Designed by: Alexander Schanne   Group: iGEM19_UZurich   (2019-10-19)


pSEVA44.1

This is the backbone of a low copy number plasmid that is optimized for transient expression of genes in Pseudomonas chlororaphis. It works also well for protein expression in E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 573
    Illegal XbaI site found at 600
    Illegal SpeI site found at 662
    Illegal PstI site found at 612
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 573
    Illegal SpeI site found at 662
    Illegal PstI site found at 612
    Illegal NotI site found at 563
    Illegal NotI site found at 630
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 573
    Illegal BamHI site found at 594
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 573
    Illegal XbaI site found at 600
    Illegal SpeI site found at 662
    Illegal PstI site found at 612
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 573
    Illegal XbaI site found at 600
    Illegal SpeI site found at 662
    Illegal PstI site found at 612
    Illegal NgoMIV site found at 1649
    Illegal NgoMIV site found at 2051
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
Parameters
None