Part:BBa_K3265029
gp88-egfp (Phage201-phi2-1 protein fusion to egfp)
The function of this protein has yet to be discovered but our results show that when a Pseudomonas bacteria is infected with the phage 201 phi2-1 two concentric circles appear at both poles of the bacteria which might indicate they might be involved in spindle pole formation. The spindle poles attached to the phuZ like proteins are what help the structure move to the center and keep the structure in place.
Background and Biology
Jumbo ϕKZ-like phages have been the object of many studies in the recent years. This is manly because of the fact that they are bacteriophages of the very lethal, opportunistic and multi antibiotic resistant bacteria Pseudomonas aeruginosa. The phages themselves not being harmfull to humans where considered in phage therapies for illneses that range from the serious lung infections to the less serious "swimmers ear".
During the rave of ϕKZ-like phage discovery and characterization, a new member of the genus was discovered : 201 phi2-1. This virus, although it infects a non pathogenic member of the Pseudomonas family (Pseudomonas Chlororaphis),it still garnered quite a bit of interest from the scientific community.
This interest was based on the fact that when pseudomonas chlororaphis is infected by the 201 phi2-1, a certain amount of the infected population starts showing a nucleus like structure approximately 40min psi (post infection). This nucleus like structure was then identified to be a compartment made by the virus to protect its self from the bacterias natural defences. Crisper cas9 enzymes were shown to have no effect against viral dna when it was in the compartement (insert relevant papers). The compartment was also shown to functionally separate transcription and translation, which forced researchers to belive that this compartment had a way to let transcripts out and let newly translated proteins in. Viral capsids after translation and self assembly are somehow docked onto the outer layer of said compartement, soon after packed with viral protein and dna.
Characterization
Usage
Visualizing the phages compartement.
Part overview with snapviewer
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 139
Illegal NotI site found at 1633
Illegal NotI site found at 4221 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2438
Illegal BglII site found at 2899
Illegal BglII site found at 3674
Illegal BamHI site found at 1658 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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