DNA

Part:BBa_K3265028

Designed by: Roy Hove   Group: iGEM19_UZurich   (2019-10-18)


gp287-egfp (Phage201-phi2-1 protein fusion to egfp)

gp287 is the phage 201 phi2-1s RecA or Uvsx like protein that is proposed to help the in transcriptional functions.


Biology

gp287 being a RecA homologous protein. It being a RecA homologue we propose that it takes part in DNA repair of the Phages DNA in case the native bacterial endonucleases do damage before the phage DNA has been compartmentalized in the pseudonucleus. Imaging shows perfect colocalization, which means it has direct access to the phage DNA, does not necessarily prove its proposed function.

800px-T--UZurich--gp287_1.jpg

Image of colocalization 40psi


Part overview in snapviewer

800px-T--UZurich--gp287snapview.png


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 149
    Illegal EcoRI site found at 1643
    Illegal XbaI site found at 2397
    Illegal SpeI site found at 3542
    Illegal PstI site found at 3492
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 149
    Illegal EcoRI site found at 1643
    Illegal SpeI site found at 3542
    Illegal PstI site found at 3492
    Illegal NotI site found at 139
    Illegal NotI site found at 1633
    Illegal NotI site found at 3510
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 149
    Illegal EcoRI site found at 1643
    Illegal BamHI site found at 1658
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 149
    Illegal EcoRI site found at 1643
    Illegal XbaI site found at 2397
    Illegal SpeI site found at 3542
    Illegal PstI site found at 3492
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 149
    Illegal EcoRI site found at 1643
    Illegal XbaI site found at 2397
    Illegal SpeI site found at 3542
    Illegal PstI site found at 3492
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
biologyTest