Composite

Part:BBa_K3260010

Designed by: Dominique WuDunn   Group: iGEM19_BrownStanfordPrinctn   (2019-09-14)


6x His hG-CSF with EK site in pet23b

This composite part consists of a T7 promoter with RBS (BBa_K3260005), followed by a 6x N-Terminus His Tag (BBa_K3260003), followed by a coding region for the solubility enhancer PpiB (BBa_K3260000), an EK cleavage site (BBa_K3260009), and hG-CSF (BBa_K3260001), followed by a stop codon, spacer, and T7 terminator (BBa_K3260006).

This construct produces a fusion protein with an N-Terminus his tag, the solubility enhancer PpiB, a TEV cleavage site, and the protein hG-CSF.

The his tag allows for his tag purification, the PpiB increases solubility, EK is a site that allows you to cleave the hG-CSF from the PpiB and his tag using a protease, and hG-CSF is a drug used to stimulate white blood cell growth.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 39
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 39
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 39
    Illegal AgeI site found at 356
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 561
    Illegal BsaI.rc site found at 20
    Illegal SapI site found at 304


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Categories
Parameters
None