Composite

Part:BBa_K3259003

Designed by: Tsuruta Soh   Group: iGEM19_TokyoTech   (2019-10-15)


Contructs for characterization of pCold-Rhl hybrid promoter


This construct was designed to quantify BBa_K325901. BBa_K3259004 is expected to be expressed by adding PAI2(C4-HSL) at low temperature condition.

This construct was made by combining GFP-containing part located at the downstream of BBa_K3259001 and other part that constitutively produces RhlR.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1079
    Illegal NheI site found at 1102
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1373
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1848
    Illegal BsaI.rc site found at 988


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Categories
Parameters
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