Regulatory

Part:BBa_K3259000

Designed by: Tsuruta Soh   Group: iGEM19_TokyoTech   (2019-10-15)


pYcgZ-Rhl hybrid promoter

This promoter consists of blue light sensitive promoter pYcg (BBa_K909010) with a RhlR / PAI2(C4-HSL) complex binding site upstream.

pYcg is repressed by the BluR repressor, but is induced by the binding of BluR to BluF protein when exposed to blue light at 470 nm. RhlO is derived from BBa_R0071 and is induced by the binding of RhlR / PAI2 onto it.

This hybrid promoter is expected to be controlled by two factors: inducer and environmental factors.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None