Composite

Part:BBa_K3258029

Designed by: Ayush Pandit   Group: iGEM19_Stanford   (2019-10-21)


MDV + CAM Flipped with Terminator

MDV + CAM Flipped (BBa_K3258028) with an additional terminator region to guarantee that stray promoters on the opposite antisense strand do not result in the production of sense CAM resistance gene RNA, to make sure the construct can be used to test the functionality of the Qbeta system for the replication of target genes flanked with MDV regions.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 180
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 146
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 172
    Illegal XhoI site found at 168
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 180
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 180
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None