Coding

Part:BBa_K3258021

Designed by: Ayush Pandit   Group: iGEM19_Stanford   (2019-10-20)


Ampicillin resistance gene with 2 stop codons from A to T at 32 and 79

This is the standard ampicillin resistance gene with 2 stop codon point mutations from an A at 32 to T and an A at 79 to T. Can be used as a test construct for the viability of directed evolution techniques to select for re-evolution of ampicillin resistance functionality.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None