Plasmid

Part:BBa_K3258014

Designed by: Alec Lourenco   Group: iGEM19_Stanford   (2019-10-16)


Selection Plasmid T7 to T3 PREDCEL+ (Alternate Version)

Selection plasmid for PREDCEL+ assembled within a pLITMUS-derived phagemid instead of a pET24a+ backbone. T7 polymerase is again present as the gene to evolve.

Refer to parts BBa_K3258009 and BBa_K3258013 for further information.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5580
    Illegal PstI site found at 1740
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5580
    Illegal NheI site found at 2806
    Illegal NheI site found at 2829
    Illegal NheI site found at 5811
    Illegal NheI site found at 5834
    Illegal PstI site found at 1740
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5580
    Illegal BglII site found at 1728
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5580
    Illegal PstI site found at 1740
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5580
    Illegal PstI site found at 1740
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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