Plasmid
Part:BBa_K3258013
Designed by: Alec Lourenco Group: iGEM19_Stanford (2019-10-16)
Selection Plasmid T7 to T3 PREDCEL+
Selection plasmid for PREDCEL+ (refer to BBa_K3258009) with T7 polymerase substituted for mRFP.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 5409
Illegal EcoRI site found at 7798
Illegal EcoRI site found at 7928
Illegal PstI site found at 1379 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 5409
Illegal EcoRI site found at 7798
Illegal EcoRI site found at 7928
Illegal NheI site found at 5421
Illegal NheI site found at 5444
Illegal PstI site found at 1379
Illegal NotI site found at 69 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 5409
Illegal EcoRI site found at 7798
Illegal EcoRI site found at 7928
Illegal BglII site found at 5333
Illegal BamHI site found at 5403
Illegal XhoI site found at 78
Illegal XhoI site found at 7205 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 5409
Illegal EcoRI site found at 7798
Illegal EcoRI site found at 7928
Illegal PstI site found at 1379 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 5409
Illegal EcoRI site found at 7798
Illegal EcoRI site found at 7928
Illegal PstI site found at 1379
Illegal NgoMIV site found at 377
Illegal NgoMIV site found at 3553
Illegal NgoMIV site found at 3713
Illegal NgoMIV site found at 5301
Illegal NgoMIV site found at 7481 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |