Plasmid

Part:BBa_K3258013

Designed by: Alec Lourenco   Group: iGEM19_Stanford   (2019-10-16)


Selection Plasmid T7 to T3 PREDCEL+

Selection plasmid for PREDCEL+ (refer to BBa_K3258009) with T7 polymerase substituted for mRFP.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5409
    Illegal EcoRI site found at 7798
    Illegal EcoRI site found at 7928
    Illegal PstI site found at 1379
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5409
    Illegal EcoRI site found at 7798
    Illegal EcoRI site found at 7928
    Illegal NheI site found at 5421
    Illegal NheI site found at 5444
    Illegal PstI site found at 1379
    Illegal NotI site found at 69
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5409
    Illegal EcoRI site found at 7798
    Illegal EcoRI site found at 7928
    Illegal BglII site found at 5333
    Illegal BamHI site found at 5403
    Illegal XhoI site found at 78
    Illegal XhoI site found at 7205
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5409
    Illegal EcoRI site found at 7798
    Illegal EcoRI site found at 7928
    Illegal PstI site found at 1379
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5409
    Illegal EcoRI site found at 7798
    Illegal EcoRI site found at 7928
    Illegal PstI site found at 1379
    Illegal NgoMIV site found at 377
    Illegal NgoMIV site found at 3553
    Illegal NgoMIV site found at 3713
    Illegal NgoMIV site found at 5301
    Illegal NgoMIV site found at 7481
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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