Composite

Part:BBa_K3258012

Designed by: Alec Lourenco   Group: iGEM19_Stanford   (2019-10-16)


lacI-derived biosensor selection schema (negative selection) PREDCEL+

This composite encodes a selection schema for evolving lacI to either bind to a novel substrate or bind to existing ones with altered affinity using the Stanford 2019 iGEM's PREDCEL+. The strategy involves both a positive and negative selection step, which the user alternates between to conduct the evolution.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 240
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 115
    Illegal BamHI site found at 1028
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 240
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 240
    Illegal NgoMIV site found at 13
    Illegal AgeI site found at 997
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None