Composite

Part:BBa_K3258010

Designed by: Alec Lourenco   Group: iGEM19_Stanford   (2019-10-16)


T7 to T3 selection schema PREDCEL+

This composite encodes a selection schema for evolving the T7 polymerase to bind to a T3 promoter using the Stanford 2019 iGEM's PREDCEL+.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 146
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 143
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 146
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 146
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None