Plasmid

Part:BBa_K3258008

Designed by: Alec Lourenco   Group: iGEM19_Stanford   (2019-10-16)


M13 Helper Plasmid for PREDCEL+

This plasmid contains a p15a origin of replication and kanamycin resistance and encodes all genes necessary to produce M13 coliphage minus a few key genes listed below:

gene III (expresses pIII) - This protein confers resistance to phage infection if expressed. gene IV (expresses pIV) - pIV production leads to cell phage shock response. gene VI (expresses pVI) - In PREDCEL+, expression of this gene is linked to increased phage production. Thus, it is linked to the function of interest on a separate plasmid.

Note: this is not a phagemid (lacks M13 ori) so it will not be packaged in phage

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2047
    Illegal XhoI site found at 5314
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3583
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4288


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Categories
Parameters
None