Composite
Part:BBa_K3257110
Designed by: Yi Qiao Group: iGEM19_Fudan-TSI (2019-10-15)
gag-Q-pol with LacUV5 Promoter-IPTG Inducible
This part is an expression composite of the Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT) and LacUV5 Promoter. Under different regulation by different promoters, the expression level varies accordingly.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 236
Illegal BglII site found at 1348
Illegal BglII site found at 2654
Illegal BglII site found at 3886
Illegal BamHI site found at 2671
Illegal BamHI site found at 2977
Illegal XhoI site found at 1002 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1887
Illegal AgeI site found at 3458 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |