Composite

Part:BBa_K3257110

Designed by: Yi Qiao   Group: iGEM19_Fudan-TSI   (2019-10-15)


gag-Q-pol with LacUV5 Promoter-IPTG Inducible

This part is an expression composite of the Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT) and LacUV5 Promoter. Under different regulation by different promoters, the expression level varies accordingly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 236
    Illegal BglII site found at 1348
    Illegal BglII site found at 2654
    Illegal BglII site found at 3886
    Illegal BamHI site found at 2671
    Illegal BamHI site found at 2977
    Illegal XhoI site found at 1002
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1887
    Illegal AgeI site found at 3458
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None